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LUKASZ LEBIODA , PhD
Lab
LAB PERSONNEL
Lukasz Lebioda Professor, USC
Geqing Chai Senior Graduate Student
Leslie Lovelace Graduate Student
Brittanaie Bell Graduate Student
Jie Qin Graduate Student
PAST PERSONNEL
Dr. Erli Zang (currently at Eli Lilly and Company)
Dr. Clarissa Jacob (currently at Abbott Laboratories)
Dr. Michael LaCount (owns and operates William Preston &
Assoc.)
Dr. Ramin Radfar (currently and assistant professor at
Wofford College)
Dr. Jason Phan (currently at NCI as a post doc for David
Waugh)
Dr. Eric Ortlund (current at Chapel Hill as a post doc for
Matt Redinbo)
PUBLICATIONS
Lovelace LL, Minor W, Lebioda L. Structure of human
thymidylate synthase under low-salt conditions. Acta
Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):622-7.
Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L.
Expression, purification and the 1.8 angstroms resolution
crystal structure of human neuron specific enolase. J Mol
Biol. 2004 Aug 20;341(4):1015-21.
Berger SH, Berger FG, Lebioda L. Effects of ligand binding
and conformational switching on intracellular stability of
human thymidylate synthase. Biochim Biophys Acta. 2004 Jan
14;1696(1):15-22.
Brewer JM, Glover CV, Holland MJ, Lebioda L. Enzymatic
function of loop movement in enolase: preparation and some
properties of H159N, H159A, H159F, and N207A enolases. J
Protein Chem. 2003 May;22(4):353-61.
Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L,
Lewinski K. Crystal structure of a covalent intermediate of
endogenous human arylsulfatase A. J Inorg Biochem. 2003 Aug
1;96(2-3):386-92.
Bell BJ, Watanabe L, Rios-Steiner JL, Tulinsky A, Lebioda L,
Arni RK. Structure of 2-keto-3-deoxy-6-phosphogluconate (KDPG)
aldolase from Pseudomonas putida. Acta Crystallogr D Biol
Crystallogr. 2003 Aug;59(Pt 8):1454-8.
Ortlund E, LaCount MW, Lebioda L. Crystal structures of
human prostatic acid phosphatase in complex with a phosphate
ion and alpha-benzylaminobenzylphosphonic acid update the
mechanistic picture and offer new insights into inhibitor
design. Biochemistry. 2003 Jan 21;42(2):383-9.
Ortlund E, Parker CL, Schreck SF, Ginell S, Minor W, Sodetz
JM, Lebioda L. Crystal structure of human complement protein
C8gamma at 1.2 A resolution reveals a lipocalin fold and a
distinct ligand binding site. Biochemistry. 2002 Jun
4;41(22):7030-7.
Freeman DL, Odom JD, Nutt WR, Lebioda L. Photoinduced
Reactions of Tri-tert-butylindium with (Diethylamino)trimethylstannane
and (Di-n-butylamino)trimethylstannane. Inorg Chem. 1997 Jun
18;36(13):2718-2722.
Phan J, Koli S, Minor W, Dunlap RB, Berger SH, Lebioda L.
Human thymidylate synthase is in the closed conformation
when complexed with dUMP and raltitrexed, an antifolate
drug. Biochemistry. 2001 Feb 20;40(7):1897-902.
Phan J, Steadman DJ, Koli S, Ding WC, Minor W, Dunlap RB,
Berger SH, Lebioda L. Structure of human thymidylate
synthase suggests advantages of chemotherapy with
noncompetitive inhibitors. J Biol Chem. 2001 Apr
27;276(17):14170-7.
Lebioda L. The honorary enzyme haemoglobin turns out to be a
real enzyme. Cell Mol Life Sci. 2000 Dec;57(13-14):1817-9.
Radfar R, Leaphart A, Brewer JM, Minor W, Odom JD, Dunlap RB,
Lovell CR, Lebioda L. Cation binding and thermostability of
FTHFS monovalent cation binding sites and thermostability of
N10-formyltetrahydrofolate synthetase from Moorella
thermoacetica. Biochemistry. 2000 Nov 28;39(47):14481-6.
Brewer JM, Holland MJ, Lebioda L. The H159A mutant of yeast
enolase 1 has significant activity. Biochem Biophys Res
Commun. 2000 Oct 5;276(3):1199-202.
Phan J, Mahdavian E, Nivens MC, Minor W, Berger S, Spencer
HT, Dunlap RB, Lebioda L. Catalytic cysteine of thymidylate
synthase is activated upon substrate binding. Biochemistry.
2000 Jun 13;39(23):6969-78.
LaCount MW, Zhang E, Chen YP, Han K, Whitton MM, Lincoln DE,
Woodin SA, Lebioda L. The crystal structure and amino acid
sequence of dehaloperoxidase from Amphitrite ornata indicate
common ancestry with globins. J Biol Chem. 2000 Jun
23;275(25):18712-6.
Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD,
Dunlap RB, Lebioda L. The crystal structure of
N(10)-formyltetrahydrofolate synthetase from Moorella
thermoacetica. Biochemistry. 2000 Apr 11;39(14):3920-6.
Ortlund E, Lacount MW, Lewinski K, Lebioda L. Reactions of
Pseudomonas 7A glutaminase-asparaginase with diazo analogues
of glutamine and asparagine result in unexpected covalent
inhibitions and suggests an unusual catalytic triad
Thr-Tyr-Glu. Biochemistry. 2000 Feb 15;39(6):1199-204.
Fantz C, Shaw D, Jennings W, Forsthoefel A, Kitchens M, Phan
J, Minor W, Lebioda L, Berger FG, Spencer HT. Drug-resistant
variants of Escherichia coli thymidylate synthase: effects
of substitutions at Pro-254. Mol Pharmacol. 2000
Feb;57(2):359-66.
Jakob CG, Lewinski K, Kuciel R, Ostrowski W, Lebioda L.
Crystal structure of human prostatic acid phosphatase .
Prostate. 2000 Feb 15;42(3):211-8.
Lebioda L, LaCount MW, Zhang E, Chen YP, Han K, Whitton MM,
Lincoln DE, Woodin SA. An enzymatic globin from a marine
worm. Nature. 1999 Sep 30;401(6752):445.
LaCount MW, Handy G, Lebioda L. Structural origins of L(+)-tartrate
inhibition of human prostatic acid phosphatase. J Biol Chem.
1998 Nov 13;273(46):30406-9.
Haeseleer F, Huang J, Lebioda L, Saari JC, Palczewski K.
Molecular characterization of a novel short-chain
dehydrogenase/reductase that reduces all-trans-retinal. J
Biol Chem. 1998 Aug 21;273(34):21790-9.
Brewer JM, Glover CV, Holland MJ, Lebioda L. Significance of
the enzymatic properties of yeast S39A enolase to the
catalytic mechanism. Biochim Biophys Acta. 1998 Apr
2;1383(2):351-5.
Lovelace L, Lewinski K, Jakob CG, Kuciel R, Ostrowski W,
Lebioda L. Prostatic acid phosphatase: structural aspects of
inhibition by L-(+)-tartrate ions. Acta Biochim Pol.
1997;44(4):673-8.
Zhang E, Brewer JM, Minor W, Carreira LA, Lebioda L.
Mechanism of enolase: the crystal structure of asymmetric
dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate
at 2.0 A resolution. Biochemistry. 1997 Oct
14;36(41):12526-34.
Brewer JM, Glover CV, Holland MJ, Lebioda L. Effect of
site-directed mutagenesis of His373 of yeast enolase on some
of its physical and enzymatic properties. Biochim Biophys
Acta. 1997 Jun 20;1340(1):88-96.
Jakob CG, Lewinski K, LaCount MW, Roberts J, Lebioda L. Ion
binding induces closed conformation in Pseudomonas 7A
glutaminase-asparaginase (PGA): crystal structure of the
PGA-SO4(2-)-NH4+ complex at 1.7 A resolution. Biochemistry.
1997 Jan 28;36(4):923-31.
Zhang E, Chen YP, Roach MP, Lincoln DE, Lovell CR, Woodin
SA, Dawson JH, Lebioda L. Crystallization and initial
spectroscopic characterization of the heme-containing
dehaloperoxidase from the marine polychaete Amphitrite
ornata. Acta Crystallogr D Biol Crystallogr. 1996 Nov
1;52(Pt 6):1191-3.
Boles JO, Lewinski K, Kunckle MG, Hatada M, Lebioda L,
Dunlap RB, Odom JD. Expression, characterization and
crystallographic analysis of telluromethionyl dihydrofolate
reductase. Acta Crystallogr D Biol Crystallogr. 1995 Sep
1;51(Pt 5):731-9.
Sangadala VS, Glover CV, Robson RL, Holland MJ, Lebioda L,
Brewer JM. Preparation by site-directed mutagenesis and
characterization of the E211Q mutant of yeast enolase 1.
Biochim Biophys Acta. 1995 Aug 16;1251(1):23-31.
Hilal SH, Brewer JM, Lebioda L, Carreira LA. Calculated
effects of the chemical environment of 2-phospho-D-glycerate
on the pKa of its carbon-2 and correlations with the
proposed mechanism of action of enolase. Biochem Biophys Res
Commun. 1995 Jun 15;211(2):607-13.
Wang S, Scott RA, Lebioda L, Zhou ZH, Brewer JM. An X-ray
absorption spectroscopy study of the interactions of Ni2+
with yeast enolase. J Inorg Biochem. 1995 May
15;58(3):209-21.
Boles JO, Lebioda L, Dunlap RB, Odom JD. Telluromethionine
in structural biochemistry. SAAS Bull Biochem Biotechnol.
1995;8:29-34.
Zhang E, Hatada M, Brewer JM, Lebioda L. Catalytic metal ion
binding in enolase: the crystal structure of an
enolase-Mn2+-phosphonoacetohydroxamate complex at 2.4-A
resolution. Biochemistry. 1994 May 24;33(20):6295-300.
Zhang E, Lebioda L, Chen Y, Brewer JM, Hatada M, Minor W.
Crystallization at low salt concentration and alkaline pH
and preliminary crystallographic data for a monoclinic form
of yeast enolase. Acta Crystallogr D Biol Crystallogr. 1994
May 1;50(Pt 3):335-6.
Boles JO, Lewinski K, Kunkle M, Odom JD, Dunlap B, Lebioda
L, Hatada M. Bio-incorporation of telluromethionine into
buried residues of dihydrofolate reductase. Nat Struct Biol.
1994 May;1(5):283-4.
Brewer JM, Robson RL, Glover CV, Holland MJ, Lebioda L.
Preparation and characterization of the E168Q site-directed
mutant of yeast enolase 1. Proteins. 1993 Dec;17(4):426-34.
Lebioda L, Zhang E, Lewinski K, Brewer JM. Fluoride
inhibition of yeast enolase: crystal structure of the
enolase-Mg(2+)-F(-)-Pi complex at 2.6 A resolution.
Proteins. 1993 Jul;16(3):219-25.
Palczewski K, Buczylko J, Lebioda L, Crabb JW, Polans AS.
Identification of the N-terminal region in rhodopsin kinase
involved in its interaction with rhodopsin. J Biol Chem.
1993 Mar 15;268(8):6004-13.
Lewinski K, Hui Y, Jakob CG, Lovell CR, Lebioda L.
Crystallization and preliminary crystallographic data for
formyltetrahydrofolate synthetase from Clostridium
thermoaceticum. J Mol Biol. 1993 Feb 20;229(4):1153-6.
Lebioda L, Stec B. Mapping of isozymic differences in
enolase. Int J Biol Macromol. 1991 Apr;13(2):97-100.
Lebioda L, Stec B, Brewer JM, Tykarska E. Inhibition of
enolase: the crystal structures of enolase-Ca2(+)-
2-phosphoglycerate and enolase-Zn2(+)-phosphoglycolate
complexes at 2.2-A resolution. Biochemistry. 1991 Mar
19;30(11):2823-7.
Lebioda L, Stec B. Mechanism of enolase: the crystal
structure of enolase-Mg2(+)-2-phosphoglycerate/phosphoenolpyruvate
complex at 2.2-A resolution. Biochemistry. 1991 Mar
19;30(11):2817-22.
Lebioda L, Hargrave PA, Palczewski K. X-ray crystal
structure of sangivamycin, a potent inhibitor of protein
kinases. FEBS Lett. 1990 Jun 18;266(1-2):102-4.
Tykarska E, Lebioda L, Marchut E, Steczko J, Stec B.
Crystallization of alcohol oxidase from Pichia pastoris.
Secondary structure predictions indicate a domain with the
eightfold beta/alpha-barrel fold. J Protein Chem. 1990
Feb;9(1):83-6.
Stec B, Lebioda L. Refined structure of yeast apo-enolase at
2.25 A resolution. J Mol Biol. 1990 Jan 5;211(1):235-48.
Lebioda L, Stec B, Brewer JM. The structure of yeast enolase
at 2.25-A resolution. An 8-fold beta + alpha-barrel with a
novel beta beta alpha alpha (beta alpha)6 topology. J Biol
Chem. 1989 Mar 5;264(7):3685-93.
Lebioda L, Stec B. Crystal structure of enolase indicates
that enolase and pyruvate kinase evolved from a common
ancestor. Nature. 1988 Jun 16;333(6174):683-6.
Tykarska E, Lebioda L, Bradshaw TP, Dunlap RB.
Crystallization and crystallographic data for new forms of
thymidylate synthase from Lactobacillus casei. J Mol Biol.
1986 Sep 5;191(1):147-50.
Lebioda L, Brewer JM. Crystallization and preliminary
crystallographic data for a tetragonal form of yeast
enolase. J Mol Biol. 1984 Nov 25;180(1):213-5.
Lebioda L, Hatada MH, Tulinsky A, Mavridis IM. Comparison of
the folding of 2-keto-3-deoxy-6-phosphogluconate aldolase,
triosephosphate isomerase and pyruvate kinase. Implications
in molecular evolution. J Mol Biol. 1982 Dec
5;162(2):445-58.
Mavridis IM, Hatada MH, Tulinsky A, Lebioda L. Structure of
2-keto-3-deoxy-6-phosphogluconate aldolase at 2 . 8 A
resolution. J Mol Biol. 1982 Dec 5;162(2):419-44.
Krajewski JW, Urbanczyk-Lipkowska Z, Gluzinski P, Lebioda L,
Busko-Oszczapowicz I, Cieslak J. The X-ray structural
investigation of
6-(n,n-1,6-hexyleneformamidine)-penicillanic acid
(mecillinam). J Antibiot (Tokyo). 1981 Mar;34(3):282-7.
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